mouse monoclonal anti cd209 antibody  (R&D Systems)

Journal: iScience

Article Title: Macrophages treated with interferons induce different responses in lymphocytes via extracellular vesicles

doi: 10.1016/j.isci.2024.109960

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti CD209/DC-SIGN (clone REA617) Conjugated APC , Miltenyi , Cat#130-124-257.

Techniques: Recombinant, Protein Extraction, Protease Inhibitor, Pore Size, Electrophoresis, Staining, Software, Cytometry

Journal: International journal of molecular sciences

Article Title: Analysis of CD1a-Positive Monocyte-Derived Cells in the Regional Lymph Nodes of Patients with Gallbladder Cancer.

doi: 10.3390/ijms252312763

Figure Lengend Snippet: Figure 2. Representative images of immunohistochemistry of CD209 (DC-SIGN) ((a) original mag- nification ×100, (b) original magnification ×200). (a) Many CD209-positive cells are found at the sinuses of LNs. (b) In metastatic LNs, CD209-positive cells are found at stroma surrounding cancer cells, some of them having dendritic shapes. (c) Image of immunohistochemistry of CD1a, almost the same area of (b). Dendritic-shaped CD1a-positive cells are found at stroma surrounding cancer cells (original magnification ×200).

Article Snippet: The following primary antibodies were used: the mouse monoclonal anti-CD1a antibody (clone 010; IS06930–2; prediluted; Dako, Glostrup, Denmark); the rabbit polyclonal antiS100 antibody (GA50461–2 J; prediluted; Dako); and the mouse monoclonal anti-CD209 antibody (clone 120507; R&D systems, Minneapolis, MN, USA).

Techniques: Immunohistochemistry

Journal: Parasite immunology

Article Title: Brugia malayi infective larvae fail to activate Langerhans cells and dermal dendritic cells in human skin

doi: 10.1111/pim.12169

Figure Lengend Snippet: Dendritic cell subset phenotypes and distribution in human skin. (a) Representative flow cytometric staining of total cells from human skin to separate CD14+ DDC, LC, CD1c+ DC and CD141+ DC. Gates were drawn based on fluorescence minus one (FMO) and unstained conditions. All final populations were also HLA-DR+. The distribution of CD14+ DDC, LC, CD1c+ DC and CD141+ DC are shown as percentages of total skin cells in (b). Each dot represents an independent donor, and the bars indicate the geometric mean (n = 7). Total skin cells were also exposed to 5 L3 or PolyI:C, and the distribution of dermal dendritic cell subsets as a percentage of total skin cells, compared to untreated skin cells, is shown in (c). Each line represents an independent donor (n = 7).

Article Snippet: Antibodies characterizing DDC included the following: APC-eFluor 780-conjugated CD14, FITC-conjugated mouse anti-human CD209 (BD Biosciences), rat anti-human CX3CR1 conjugated to APC (eBioscience), mouse anti-human CD11c conjugated to Brilliant Violet 650 (BioLegend), FITC-conjugated mouse anti-human CD1a (BD Biosciences), mouse anti-human CD141 conjugated to PE-Cy7 (Biolegend), PerCP-eFluor 710-conjugated mouse anti-human CD1c (eBioscience), eFluor 450-conjugated rat anti-human IL-10 (eBioscience), Alexa Fluor 700-conjugated rat anti-human IL-6 (eBioscience), Alexa Fluor 700-conjugated mouse anti-human TNF-α (Biolegend) and mouse anti-human CCL22 conjugated to PE (R&D).

Techniques: Staining, Fluorescence

Journal: Parasite immunology

Article Title: Brugia malayi infective larvae fail to activate Langerhans cells and dermal dendritic cells in human skin

doi: 10.1111/pim.12169

Figure Lengend Snippet: Inflammatory, regulatory and alternative cytokine production by skin dendritic cells. Flow cytometric analysis of CD14+ DDC, LC, CD1c+ DC and CD141+ DC from total skin included intracellular cytokine staining for (a) IL-6, (b) IL-10 and (c) CCL22. Total skin cells were exposed to 5 L3 or PolyI:C (25 mg/mL) for 24 h. Data are shown as percentage of cells in each DC subset expressing the indicated cytokine. Positive and negative gates were determined by FMO and unstained conditions. Each line represents an independent donor, and P-values were calculated by Wilcoxon matched-pairs signed rank test (n = 7).

Article Snippet: Antibodies characterizing DDC included the following: APC-eFluor 780-conjugated CD14, FITC-conjugated mouse anti-human CD209 (BD Biosciences), rat anti-human CX3CR1 conjugated to APC (eBioscience), mouse anti-human CD11c conjugated to Brilliant Violet 650 (BioLegend), FITC-conjugated mouse anti-human CD1a (BD Biosciences), mouse anti-human CD141 conjugated to PE-Cy7 (Biolegend), PerCP-eFluor 710-conjugated mouse anti-human CD1c (eBioscience), eFluor 450-conjugated rat anti-human IL-10 (eBioscience), Alexa Fluor 700-conjugated rat anti-human IL-6 (eBioscience), Alexa Fluor 700-conjugated mouse anti-human TNF-α (Biolegend) and mouse anti-human CCL22 conjugated to PE (R&D).

Techniques: Staining, Expressing

Journal: iScience

Article Title: Derived myeloid lineage induced pluripotent stem as a platform to study human C-C chemokine receptor type 5Δ32 homozygotes

doi: 10.1016/j.isci.2023.108331

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Article Snippet: PE-conjugated Mouse monoclonal antibody anti-human CD209 (Clone: DCN46) , BD , Cat# 551265; RRID: AB_394123.

Techniques: Virus, Recombinant, Modification, Purification, Blocking Assay, Luminex, Pore Size, Amplification, Software

Journal: STAR Protocols

Article Title: In vitro expansion of Wilms’ tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells

doi: 10.1016/j.xpro.2023.102053

Figure Lengend Snippet: Antibody staining panels for flow cytometric analysis of mature IL-4 DC phenotype on a CytoFlex cytometer

Article Snippet: Mouse anti-human CD209 - FITC - Clone DCN46 (3 μL/10 5 cells) , BD Biosciences , Cat# 551264 RRID: AB_394122.

Techniques: Staining

Journal: STAR Protocols

Article Title: In vitro expansion of Wilms’ tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells

doi: 10.1016/j.xpro.2023.102053

Figure Lengend Snippet:

Article Snippet: Mouse anti-human CD209 - FITC - Clone DCN46 (3 μL/10 5 cells) , BD Biosciences , Cat# 551264 RRID: AB_394122.

Techniques: Produced, Recombinant, Staining, Software, Flow Cytometry